Enzymatic Hydrolysis and Synthesis of Benzohydroxamic Acid.

Synthesis and enzymatic hydrolysis of glutamic acid polypeptides.

Synthetic lysine polypeptides of high molecular weight (1) have been shown to reduce virus infectivity (2, 3), inhibit bacterial growth and respiration (4), agglutinate virus particles, bacterial cells, and erythrocytes (4-6), and to prolong blood clotting time (7). It has been suggested that most of these phenomena are associated with the formation of many ionic bonds between the positively ch...

Enzymatic Synthesis and Hydrolysis of Linear Alkyl and Steryl Hydroxycinnamic Acid Esters

The synthesis and enzymatic hydrolysis of poly-D-lysine.

In studies of the inhibition in viva of animal viruses by poly-n-lysine, Rubini et al. (1) and Green et al. (2-4) obtained data which suggested that poly-L-lysine is hydrolyzed by the proteolytic enzymes of the allantoic fluid, and that this enzymatic hydrolysis may limit its biological activity. The enzymatic hydrolysis of poly-n-lysine was reported by Katchalski (5) and by Waley and Watson (6...

Microbial and enzymatic hydrolysis of poly(aspartic acid)

The microbial and enzymatic hydrolysis of poly(aspartic acid) (PAA) has been reviewed. Two PAA-degrading bacteria (Pedobacter sp. KP-2 and Sphingomonas sp. KT-1) were isolated from flesh river water. Pedobacter sp. KP-2 hydrolyzed PAA of high molecular weights over 5000. Sphingomonas sp. KT-1 hydrolyzed only PAA of low molecular weights (<5000), while the cell extract could hydrolyze high-molec...

Enzymatic synthesis of desoxyribonucleic acid.

Double stranded DNA serves as a template primer for Escherichia coli DNA polymerase when the DNA contains a single strand break (a nick) with a 3’-hydroxyl terminus. Initiation of replication entails covalent extension of the 3’-hydroxyl terminus and a concurrent 5’ -+ 3’ nuclease action by the enzyme. The primer strand is hydrolyzed at the 5‘ side of the nick while the synthetic activity catal...